Protective role of down-regulated microRNA-31 on intestinal barrier dysfunction through inhibition of NF-κB/HIF-1α pathway by binding to HMOX1 in rats with sepsis

Table 1 RT-qPCR primer sequences

mRNA	Forward primers (5′-3′)	Reverse primers (5′-3′)
miR-31	CAGCTATGCCAGCATCTTGCCT	ATATGGAACGCTTCACGAATT
NF-κB	GGGCATGGGAATTTCCAACTC	GCAGAAGTAACTTTCCGAGAGG
IκB	TTGCTGAGGCACTTCTGAAAG	TCTGCGTCAAGACTGCTACAC
HIF-1α	GTCGGACAGCCTCACCAAACAG	TAGGTAGTGAGCCACCAGTGTCC
HMOX-1	CTCAAACCTCCAAAAGCC	TCAAAAACCACCCCAACCC
ZO-1	CCATTCTTTGGACCGATTGCTG	TAATGCCCGAGCTCCGATG
Occludin	ACGGTGCCATAGAATGAGATGTTG	CAGCTAGTTGTTCATTTCTGCACCA
GADPH	TTCACCACCATGGAGAAGGC	GGCATGGACTGTGGTCATGA
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT

Note: RT-qPCR Reverse transcription quantitative polymerase chain reaction, miR-31 microRNA-31, NF-κB nuclear factor-kappa B, IκB inhibitor of NF-κB, HIF-1α hypoxia inducible factor-1α, HMOX-1 heme oxygenase 1, ZO-1 zonula occludens-1, GADPH glyceraldehyde-3-phosphate dehydrogenase

ISSN: 1528-3658