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Fig. 2 | Molecular Medicine

Fig. 2

From: Propofol inhibited autophagy through Ca2+/CaMKKβ/AMPK/mTOR pathway in OGD/R-induced neuron injury

Fig. 2

PPF antagonized OGD/R-triggered cell injury, autophagy induction and [Ca2+]i elevation. Propofol (PPF) with various concentrations (0.01 mM, 0.03 mM, 0.05 mM in 0.1% DMSO) was added into OGD/R-exposed neurons. a LDH release. b Cell viability using MTT assay. c, d Western blot analysis of protein expression of autophagy-related genes LC3, Beclin1, and p62. Quantification of western blots was shown. e Ca2+ fluorescence images. Scale bar: 50 μm. The qualification of Ca2+- fluorescence intensity was shown. f The autophagosome puncta of GFP-LC3 by immunofluorescence. Scale bar: 10 μm. Values are represented as the mean ± SD from three independent experiments. *p < 0.05 vs. Control group; #p < 0.05 vs. DMSO group

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