Fig. 4
From: Propofol inhibited autophagy through Ca2+/CaMKKβ/AMPK/mTOR pathway in OGD/R-induced neuron injury
[Ca2+]i elevation abrogated the PPF-mediated amelioration of OGD/R-triggered cell injury. OGD/R-exposed neurons were pre-treated with a Ca2+ ionophore ionomycin (iono, 0.3 μM), followed by propofol (PPF, 30 μM in 0.1% DMSO) treatment. a Ca2+ fluorescence images. Scale bar: 50 μm. The qualification of Ca2+- fluorescence intensity was shown. b LDH release, c CCK-8 cell viability and d cell apoptosis assay were performed to evaluate whether [Ca2+]i was involved in the PPF-mediated amelioration of OGD/R-triggered cell injury. Values are represented as the mean ± SD from three independent experiments. *p < 0.05 vs. Control group; #p < 0.05 vs. DMSO group; $p < 0.05 vs. PPF group