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Fig. 6 | Molecular Medicine

Fig. 6

From: Propofol inhibited autophagy through Ca2+/CaMKKβ/AMPK/mTOR pathway in OGD/R-induced neuron injury

Fig. 6

Ca2+/CaMKKβ/AMPK/mTOR signaling was involved in the PPF-mediated suppression of autophagy. a Western blot was performed to evaluate the effects of [Ca2+]i elevator ionomycin (iono) on the PPF-mediated regulation of CaMKKβ, AMPK, p-AMPK, mTOR, and p-mTOR. b Western blot was performed to investigate the effects of overexpression of CaMKKβ on the PPF-mediated regulation of CaMKKβ, AMPK, p-AMPK, mTOR, and p-mTOR. c Western blot was performed to investigate the effects of activation of the AMPK pathway by AICAR on the PPF-mediated regulation of CaMKKβ, AMPK, p-AMPK, mTOR, and p-mTOR. d Western blot was performed to investigate the effects of activation of CaMKKβ/AMPK/mTOR pathway on the PPF-mediated suppression of autophagy-related proteins. e GFP-LC3 immunofluorescence was performed to evaluate the effects of activation of activation of CaMKKβ/AMPK/mTOR pathway on the PPF-mediated suppression of autophagy level. Scale bar: 10 μm. Values are represented as the mean ± SD from three independent experiments. *p < 0.05 vs. Control group; #p < 0.05 vs. DMSO group; &p < 0.05 vs. vector group; $p < 0.05 vs. PPF group

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