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Fig. 1 | Molecular Medicine

Fig. 1

From: MicroRNAs 143 and 150 in whole blood enable detection of T-cell immunoparalysis in sepsis

Fig. 1

Differential expression of miRNA in septic T-cells. a MicroRNA Microarray analysis. Heat map showing the differentially expressed miRNAs in sepsis patients as compared to healthy controls, n = 7/7 (NC/Sepsis). RNA was isolated and miRNA array analysis was performed. Yellow colour indicates upregulation of miRNA expression, red colour indicates decreased miRNA levels. b MiRNAs in human Pan T-cells of septic patients as compared to healthy controls. Expression levels of miR-150, miR-342, miR-15a, miR-16, miR-93, miR-143, miR-223 and miR-424 in Pan T-cells of septic patients and healthy controls were measured by TaqMan miRNA assays relative to U47. Data are shown as median, 25th and 75th percentile and outliers, n = 10/20 (NC/Sepsis), performed in duplicates. Values represent expression relative to controls, *p < 0.05, **p < 0.001. Quantification cycle (Cq) values for the single miRNAs were in the range of 21 (NC) and 23 (Sepsis) for miR-150, 24 (NC) and 26 (Sepsis) for miR-342, 30 (NC) and 29 (Sepsis) for miR-15a, 23 (NC) and 22 (Sepsis) for miR-16, 30 (NC) and 28 (Sepsis) for miR-93, 34 (NC) and 29 (Sepsis) for miR-143, 26 (NC) and 22 (Sepsis) for miR-223, 36 (NC) and 34 (Sepsis) for miR-424, respectively

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