Fig. 2

T-cell immunoparalysis in sepsis. Cytokine and immune receptor expression in T-cells of septic patients as compared to healthy controls. mRNA levels of (a) IL-2, (b) IL-7R (c) ICOS, (d) IL-4, (e) IL-10 and (f) TGF-ß in Pan T-cells of septic patients and healthy controls, respectively, were measured by qPCR relative to reference genes SDHA and TBP. Data are presented as median, 25th and 75th percentile and outliers, measurements were performed in duplicates. Values represent expression relative to controls, *p < 0.05, **p < 0.001. Quantification cycle (Cq) values for the single cytokines and receptors were in the range of 34 (NC) and 36 (Sepsis) for IL-2, 24 (NC) and 25 (Sepsis) for IL7R, 32 (NC) and 33 (Sepsis) for ICOS, 35 (NC) and 32 (Sepsis) for IL-4, 35 (NC) and 33 (Sepsis) for IL-10, 25 (NC) and 24 (Sepsis) for TGF-ß, respectively