Fig. 4

Simultaneous hyper- and hypoinflammation in whole blood of septic patients. a MiRNA expression in human whole blood obtained from septic patients as compared to healthy controls. Expression levels of miR-150, miR-342, miR-15a, miR-16, miR-93, miR-143, miR-223 and miR-424 were assessed by TaqMan miRNA assays relative to U47. Data are shown as median, 25th and 75th percentile and outliers, n = 10/20 (NC/Sepsis) performed in duplicates. Values represent expression relative to controls, *p < 0.05, **p < 0.001. Quantification cycle (Cq) values for the single miRNAs were in the range of 24 (NC) and 26 (Sepsis) for miR-150, 26 (NC) and 27 (Sepsis) for miR-342, 30 (NC) and 31 (Sepsis) for miR-15a, 21 (NC) and 23 (Sepsis) for miR-16, 26 (NC) and 29 (Sepsis) for miR-93, 34 (NC) and 33 (Sepsis) for miR-143, 21 (NC and Sepsis) for miR-223, 40 (NC) and 39 (Sepsis) for miR-424, respectively. b Cytokine expression in human whole blood obtained from septic patients as compared to healthy controls. mRNA levels of IL-4, IL-1β, IL-10 and TGF-β were measured by qPCR relative to reference genes SDHA and TBP. Data are shown as median, 25th and 75th percentile and outliers, n = 10/20 (NC/Sepsis) performed in duplicates. Values represent expression relative to controls, *p < 0.05, **p < 0.001. Quantification cycle (Cq) values for the single cytokines and receptors were in the range of 32 (NC) and 35 (Sepsis) for IL-4, 27 (NC and Sepsis) for IL-1β, 35 (NC) and 32 (Sepsis) for IL-10, 24 (NC) and 25 (Sepsis) for TGF-β, respectively