Fig. 8
Effect of A30 on differentiation of embryonic stem cells into different germ layer lineages. Nanog, stemness marker; Sox1, Sox3 and Tubb3, ectodermal differentiation markers; Lhx1, mesodermal differentiation marker; Sox17, endodermal differentiation marker; T, mesendodermal differentiation marker. a mRNA expression analysis of ectodermal, mesodermal and endodermal differentiation markers in MESC-R1 derived embryoid body (EB) upon the A30 treatment for 6 days after EB formation. Self-renewal, undifferentiated MESC-R1 cells; EB, embryoid body-differentiated MESC-R1 cells; EA, embryoid body differentiated MESC-R1 cells treated with 0.25 μg/ml of A30. b mRNA expression analysis of ectodermal differentiation markers in neuroectodermal differentiated MESC-R1 cells upon the A30 treatment at different concentrations for 5 days. Self-renewal, undifferentiated MESC-R1; ND, neuroectodermal differentiated MESC-R1. A30 concentration umit is μg/ml. The symbol * represents p < 0.01 compared with EB group (a) or ND group (b) by ANOVA with Tukey’s test post-hoc for the corresponding mRNA expression (n = 3)