Fig. 7

Vitamin D exacerbates lung fibrosis in mice. a Expression of liver Cyp24a1. Values are folds of induction over control mice (treated with bleomycin vehicle: saline); veh.: vitamin D vehicle. The results presented in the figures are means ± SEM. Significance of the analysis is indicated as ***, P < 0.001. b Representative micrographs of Masson’s stain of lung sections from control mice (treated with bleomycin vehicle: saline). Scale bar: 200 μm. c Representative micrographs of Masson’s stains of lung sections of mice treated with bleomycin and vitamin D or its vehicle. Scale bar: 200 μm. d Representative macrographs of lungs from control mice and treated with bleomycin and vitamin D. Right panel: body weights of a representative experimental group of mice (n = 20). The results presented in the graphs are means ± SEM. ANOVA result (P < 0,01). Significance of the analysis post-test is indicated in the figure as *, P < 0.05 and **, P < 0.01. e Expression levels of CDKN2A from lung tissue homogenates of representing the four experimental groups. TBP (tata binding protein) was used as loading control