Fig. 8

DNA damage expression in lung tissue: expression of γH2AFX foci. a Illustration showing the staining pattern of γH2AFX. γH2AFX is typically expressed as discrete foci or as a near pan-nuclear staining when the occurrence of DD foci is massive. b Expression of γH2AFX foci in representative sections of lung tissue from control mice (treated with saline). A magnification of an image of a respiratory bronchiole is depicted in the square on the left. Arrowheads signal some of the nuclei harboring DD foci containing γH2AFX distributed throughout the respiratory bronchiole or in alveolar fields (photograph on the right). c Expression of γH2AFX foci in representative sections of lung tissue from mice treated with bleomycin or bleomycin + vehicle. Two magnifications of the images are depicted in the rectangles on the top. Arrowheads signal some of the nuclei harboring some DD foci containing γH2AFX indicative of severe DNA damage. The magnitude and distribution of the damage observed was very similar in both groups. Scale bars: 100 μm