Fig. 2
From: Loss of intestinal sympathetic innervation elicits an innate immune driven colitis
Lipopolysaccharide (LPS)-exposed bone marrow derived macrophages (BMDM) had an increased anti-inflammatory phenotype after adrenergic receptor activation. mRNA expression levels of Arginase 1 (Arg1) and nitric oxide synthase 2 (Nos2) in cell lysate of BMDM after treatment with 100 ng/ml LPS for 18 h and pre-treatment with different concentrations of norepinephrine (a) or salbutamol (b) together with propranolol, normalized for reference genes β-actin and Ubiquitin. Expression is relative to mRNA expression in BMDM not exposed to any treatment. N = 4 mice. Data is expressed as median and individual data points. We tested for statistical significant differences with a Kruskal-Wallis test and post-hoc Dunn’s test. P-value < 0.05 was considered significant. * P-value < 0.05; ** P-value ≤0.01