Fig. 4

Rag1^−/− mice showed a higher histology score after treatment with 6-OHDA. a Bodyweight of mice over time. b Colon weight as a marker of inflammation, normalized for colon length. c Protein levels of interleukin (IL)-4, IL-10, IL-17, interferon (IFN)-γ and tumour necrosis factor (TNF)-α in colon homogenates, normalized for total protein levels. d Total histology score as described in Table 3 and goblet cell depletion subscore. Representative pictures are shown of a haematoxylin and eosin (HE) staining of the colon. 2x magnification. The areas within the red boxes are examples of goblet cell depletion. The black box shows an area with normal goblet cell numbers. An enlargement is shown to the right. For more examples and details of the histology score, see also Additional file 2: Figure S2. N = 10 mice. Data is expressed as mean (a, b and c) or median (TNF-α from c and d) and individual data points (all except A where standard deviation is shown). We tested for statistical significant differences with an independent T-test or a Mann-Whitney U test. P-value < 0.05 was considered significant. * P-value < 0.05