Table 1 Mouse primers
From: Loss of intestinal sympathetic innervation elicits an innate immune driven colitis
Gene | Forward sequence | Reverse sequence |
|---|---|---|
IL-1β | GCCCATCCTCTGTGACTCAT | AGGCCACAGGTATTTTGTCG |
IL-6 | GAGTTGTGCAATGGCAATTCTG | TGGTAGCATCCATCATTTCTTTGT |
IL-10 | TGTCAAATTCATTCATGGCCT | ATCGATTTCTCCCCTGTGAA |
IL-12 | AGACCCTGCCCATTGAACTG | CGGGTCTGGTTTGATGATGTC |
TNF-α | TGGAACTGGCAGAAGAGGCACT | CCATAGAACTGATGAGAGGGAGGC |
Nos2 | TTCTGTGCTGTCCCAGTGAG | TGAAGAAAACCCCTTGTGCT |
Arg1 | CTCCAAGCCAAAGTCCTTAGAG | AGGAGCTGTCATTAGGGACATC |
IL-22 | CGGCTCATCGGGGAGAAAC | TGACTGGGGGAGCAGAACG |
Reg3γ | TCCACCTCTGTTGGGTTCAT | AAGCTTCCTTCCTGTCCTCC |
DefA | CGCAGCCATGAAGAAACTTG | GAATCAGCCTGGACCTGGAA |
Muc2 | TGAAGACCGAGATTGTGCCC | AGATGACGTTGAGCTGGGTG |
β-actin | TTCTTTGCAGCTCCTTCGTT | ATGGAGGGGAATACAGCCC |
Ubiquitin | AGCCCAGTGTTACCACCAAG | ACCCAAGAACAAGCACAAGG |
HPRT | CCTAAGATGAGCGCAAGTTGAA | CCACAGGACTAGAACACCTGCTAA |
Cyclophilin | ATGGTCAACCCCACCGTGT | TTCTGCTGTCTTTGGAACTTTGTC |
GAPDH | ATGTGTCCGTCGTGGATCTGA | ATGCCTGCTTCACCACCTTCT |
RPLP0 | CCAGCGAGGCCACACTGCTG | ACACTGGCCACGTTGCGGAC |