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Table 1 Purification of recombinant wild-type and mutant ALAS2a

From: Molecular expression, characterization and mechanism of ALAS2 gain-of-function mutants

ALAS2 enzyme

Step

Activity

Specific.activity

Yield

Fold Purification

Units

Units/mg

%

fold

Wild type

Crude extract

571,000

1130

100

1

Affinity chromatography

323,000

21,500

56

20

Gel filtration

131,000

93,500

23

83

p.Arg559His

Crude Extract

1,090,000

2090

100

1

Affinity chromatography

358,000

29,600

33

14

Gel filtration

95,600

108,000

8.8

52

p.Glu565Asp

Crude extract

1,030,000

1710

100

1

Affinity chromatography

825,000

50,200

80

29

Gel filtration

194,000

122,000

19

71

p.Arg572Cys

Crude extract

267,000

780

100

1

Affinity chromatography

143,000

8760

53

11

Gel filtration

43,500

121,000

16

155

p.Ser573Phe

Crude extract

666,000

1070

100

1

Affinity chromatography

301,000

11,400

45

11

Gel filtration

84,200

115,000

13

107

p.Tyr586Phe

Crude Extract

845,000

1650

100

1

Affinity chromatography

287,000

15,800

34

10

Gel filtration

128,000

109,000

15

65

P561X

Crude Extract

4,660,000

8990

100

1

Affinity chromatography

3,110,000

156,000

66

17

Gel Filtration

1,730,000

288,000

37

32

V562X

Crude Extract

6,410,000

13,300

100

1

Affinity chromatography

2,960,000

116,000

46

9

Gel filtration

1,330,000

402,000

21

30

H563X

Crude Extract

3,480,000

9070

100

1

Affinity chromatography

2,990,000

170,000

86

19

Gel Filtration

1,610,000

300,000

46

33

F575X

Crude Extract

1,510,000

2990

100

1

Affinity chromatography

355,000

30,500

23

10

Gel Filtration

174,000

134,000

12

45

  1. aData are averages from at least 3 independent purifications