Fig. 6

a X-gal staining in the Angpt1 over-expression and control mice. Positive staining was observed in the tubular cells 7 days after the initiation of supplement of doxycycline in the Angpt1 over-expression mice. The presence of positive staining indicates that the teteracycline responsive element promoter activity of Angpt1. The β-galactosidase activity in the over expression mice was activated. b ∆Ct (difference in the threshold cycle between Angpt1 and GAPDH) of human Angpt1 mRNA by quantitative RT-PCR analysis of control and human Angpt1 over expression mice 1 day after IRI. The ∆Ct in the control mice was much higher than that in the over expression mice indicating significant over-expression of human Angpt1 mRNA was observed in the over-expression mice (N = 20 per group) c Renal tissue human Angpt1 concentration in over-expression and control mice. The over-expression mice had higher renal human Angpt1 concentration than control mice (N = 8 for each group)