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Table 1 Primers

From: Angiopoietin 1 influences ischemic reperfusion renal injury via modulating endothelium survival and regeneration

Primers Sequence
Primers used in Genotyping PCR reaction
 Angpt1 knock out F 5’ AAC CTT TCC CTC CCC TTT TT 3’
 Angpt1 knock out R 5’ TTG GTG CTG CTG AAG AAA TG 3’
 UBC-CreErt2 F 5’-GCA AGA ACC TGA TGG ACA-3’
 UBC-CreErt2 R 5’- CTA GAG CCT GTT TTG CAC-3’
 Pax8-rtTA F 5’-CCA TGT CTA GAC TGG ACA AGA-3’
 Pax8-rtTA R 5’-CTC CAG GCC ACA TAT GAT TAG-3’
 rtTA-hAngpt1 F 5’ ATA GGA ACC AGC CTC CTC TCT 3’
 rtTA-hAngpt1 R 5’ AAG GAC ACT GTT GTT GGT GGT 3’
Primers used in quantitative RT-PCR
 Mouse GAPDH F 5’ CCT GGA GAA ACC TGC CAA GTA T 3’
 Mouse GAPDH R 5’ CAT ACC AGG AAA TGA GCT TGA CA 3’
 Human Angpt1 F 5’ TCA ATC TTT GCA CTA AAG AAG GTG T 3’
 Human Angpt1 R 5’ GTC CAA CCT CCC CCA TTG AC 3’
 Mouse Angpt1 F 5’ GGC CAC CAT GCT TGA GAT AG 3’
 Mouse Angpt1 R 5’ TTT CAA GTC GGG ATG TTT GA 3’
 Mouse TNF-α F 5’ TAG CCA GGA GGG AGA ACA GA 3’
 Mouse TNF-α R 5’ TTT TCT GGA GGG AGA TGT GG 3’
 Mouse IL-1β F 5’ CCC AAG CAA TAC CCA AAG AA 3’
 Mouse IL-1β R 5’ GCT TGT GCT CTG CTT GTG AG 3’
 Mouse MCP-1 F 5’ AGC ACC AGC CAA CTC TCA CT 3’
 Mouse MCP-1 R 5’ CGT TAA CTG CAT CTG GCT GA 3’
 Mouse IL-6 F 5’ AGA CAA AGC CAG AGT CCT TCA 3’
 Mouse IL-6 R 5’ GGT CCT TAG CCA CTC CTT CTG 3’
 Mouse CX3CR1 F 5’ GGA GAC TGG AGC CAA CAG AG 3’
 Mouse CX3CR1 R 5’ CCT GAT CCA GGG AAT GCT AA 3’
 Mouse CXCL2 F 5’ CAG ACT CCA GCC ACA CTT CA 3’
 Mouse CXCL2 R 5’ CAG TTC ACT GGC CAC AAC AG 3’
 Mouse CCL3 F 5’ ATG AAG GTC TCC ACC ACT GC 3’
 Mouse CCL3 R 5’ GAT GAA TTG GCG TGG AAT CT 3’
 Mouse CCR2 F 5’ ATT CTC CAC ACC CTG TTT CG 3’
 Mouse CCR2 R 5’ GAT TCC TGG AAG GTG GTC AA 3’
 Mouse CCL17 F 5’ AGT GGA GTG TTC CAG GGA TG 3’
 Mouse CCL17 R 5’ CTG GTC ACA GGC CGT TTT AT 3’
 Mouse Arginase 1 F 5’ AGC ACT GAG GAA AGC TGG TC 3’
 Mouse Arginase 1R 5’ CAG ACC GTG GGT TCT TCA CA 3’
 Mouse IGF-1 F 5’ GGC ATT GTG GAT GAG TGT TG 3’
 Mouse IGF-1 R 5’ TCT CCT TTG CAG CTT CGT TT 3’
 Mouse EGF F 5’ AGT CAG ACC GAA AGA GCT GTG 3’
 Mouse EGF R 5’ CCT GGG AAT TTG CAA ACA GT 3’
 Mouse HGF F 5’ CGC TAC GAA GTC TGT GAC ATT C 3’
 Mouse HGF R 5’ CAT GGG ACC TCT GTA GCT TTC AC 3’
 Mouse PDGF-A F 5’ GAT GAG GAC CTG GGC TTG 3’
 Mouse PDGF-A R 5’ GAT CAA CTC CCG GGG TAT CT 3’
 Mouse PDGF-B F 5’ CCC ACA GTG GCT TTT CAT TT 3’
 Mouse PDGF-B R 5’ GTG AAC GTA GGG GAA GTG GA 3’
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Molecular Medicine

ISSN: 1528-3658