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Table 1 Primer sequence for RT-qPCR

From: Silencing of SAA1 inhibits palmitate- or high-fat diet induced insulin resistance through suppression of the NF-κB pathway

Gene

Forward (5′-3′)

Reverse (5′-3′)

SAA1 (human)

CCAATCTCCGACCTGCTG

GCTTTGTATCCCTGCCCTGAG

Saa1 (mouse)

GGACTGCCTGACAAATACTGAG

GAGCATCTTCAGTGTTCCTAGG

IRS1 (human)

AGGATATTTAATTTGCCTCGG

AAGCGTTTGTGCATGCTCTTG

SOCS3 (human)

CCACTCTTCAGCATCTCTGT

ACTGAACCTGACCGTACAATCGTACTGGTCCAGGAACT

β-actin (human)

GCAAAGACCTGTACGCCAACA

TGCATCCTGTCGGCAATG

β-actin (mouse)

GACATGGAGAAGATCTGG

CTTTACGGATGACAACG

  1. Notes: RT-qPCR reverse transcription quantitative polymerase chain reaction, Saa1 serum amyloid A protein 1, IRS1 insulin receptor substrate 1, SOCS3 suppressor of cytokine signaling 3
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Molecular Medicine

ISSN: 1528-3658