Fig. 3

PGK1 knockdown reduces the expression of DNA repair-related proteins, methylation-related enzymes, and total cellular methylation level. a. Western blot was used to detect the expression of pro-survival proteins (Bcl-xL, Mcl-1) and pro-apoptotic protein (Bax) in Ishikawa endometrial cancer cell line. β-actin was used as a loading control. b. Semi-quantitative analysis of protein level in A. c. Western blot was used to detect the expression of pro-survival proteins (Bcl-xL, Mcl-1) and pro-apoptotic protein (Bax) in HEC1A endometrial cancer cell line. β-actin was used as a loading control. d. Semi-quantitative analysis of protein level in C. e. Western blot was used to detect the expression of DNA repair-related proteins c-JUN, FOSL1, and POLD1 in Ishikawa endometrial cancer cell line. β-actin was used as a loading control. f. Semi-quantitative analysis of protein level in A. g. Western blot was used to detect the expression of DNA repair-related proteins c-JUN, FOSL1, and POLD1 in HEC1A endometrial cancer cell line. β-actin was used as a loading control. h. Semi-quantitative analysis of protein level in C. i. Western blot was performed to determine the effect of PGK1 knockdown on the total intracellular methylation levels (amount of average methylated cytosine). j. Western blot was used to examine the effect of PGK1 knockdown on the expression of DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B) and SPARC in Ishikawa cancer cell line. β-actin was used as a loading control. k. Semi-quantitative analysis of protein level in F. l. Western blot was used to examine the effect of PGK1 knockdown on the expression of DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B) and SPARC in HEC1A endometrial cancer cell line. β-actin was used as a loading control. m. Semi-quantitative analysis of protein level in H. Data were shown as mean ± SD based on three independent experiments. *, P < 0.05, **, P < 0.01 compared with shNC group