Fig. 7From: Inhibition of HMGB1/RAGE-mediated endocytosis by HMGB1 antagonist box A, anti-HMGB1 antibodies, and cholinergic agonists suppresses inflammationTNF release induced by LPS-HMGB1 complexes in RAW 264.7 cells was reduced by box A and m2G7. RAW 264.7 cells in 96-well culture plates were used at 90% confluence. Cells were stimulated with HMGB1 or LPS alone or HMGB1+ LPS, with the addition of box A, m2G7 or mouse IgG as indicated for 16 h. TNF released was measured. N = 3 experiments. *: P < 0.05 vs. HMGB1 + LPSBack to article page