Skip to main content

Table 1 Analytical testing of tag-based NGS

From: Tag-based next generation sequencing: a feasible and reliable assay for EGFR T790M mutation detection in circulating tumor DNA of non small cell lung cancer patients

RS cfDNA input (ng) Library (pM) Median Read Cov Median Mol Cov EGFR gene variants VAF (%) LOD (%) Allele Mol Cov Tot Read Cov
HD780 (5%) 30 430 53,562 4365 E746_A750delELREA 5.81 0.05 265 62,561
V769_D770insASV 4.17 0.05 220 47,935
T790M 5.46 0.05 272 73,309
L858R 5.02 0.1 119 56,740
HD780 (1%) 30 408 27,829 3614 E746_A750delELREA 0.91 0.05 37 34,718
V769_D770insASV 0.85 0.05 34 23,487
T790M 0.91 0.05 40 37,057
L858R 1.15 0.1 25 29,792
HD780 (0.1%) 30 450 28,269 3778 E746_A750delELREA 0.14 0.05 6 35,994
V769_D770insASV 0.1 0.05 4 23,378
T790M 0.11 0.05 5 33,633
L858R 0.09 0.1 2 27,703
HD780 (0%) 30 570 32,314 2353 E746_A750delELREA 0 nd 0  
V769_D770insASV 0 nd 0  
T790M 0 nd 0  
L858R 0 nd 0  
HD780 (0.1%) 20 705 22,117 2734 E746_A750delELREA 0.03 0.05 1 29,492
V769_D770insASV 0.1 0.05 3 20,616
T790M 0.19 0.05 6 28,438
L858R 0.13 0.1 2 19,308
  1. RS Reference Standard, Median Read Cov Median Read Coverage, Median Mol Cov Median Molecular Coverage, VAF Variant Allele Frequency, LOD Limit of Detection, Allele Mol Cov Allele Molecular Coverage, Tot Read Cov Total Read Coverage, nd not detected, HD Horizon Discovery