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Table 1 Analytical testing of tag-based NGS

From: Tag-based next generation sequencing: a feasible and reliable assay for EGFR T790M mutation detection in circulating tumor DNA of non small cell lung cancer patients

RS

cfDNA input (ng)

Library (pM)

Median Read Cov

Median Mol Cov

EGFR gene variants

VAF (%)

LOD (%)

Allele Mol Cov

Tot Read Cov

HD780 (5%)

30

430

53,562

4365

E746_A750delELREA

5.81

0.05

265

62,561

V769_D770insASV

4.17

0.05

220

47,935

T790M

5.46

0.05

272

73,309

L858R

5.02

0.1

119

56,740

HD780 (1%)

30

408

27,829

3614

E746_A750delELREA

0.91

0.05

37

34,718

V769_D770insASV

0.85

0.05

34

23,487

T790M

0.91

0.05

40

37,057

L858R

1.15

0.1

25

29,792

HD780 (0.1%)

30

450

28,269

3778

E746_A750delELREA

0.14

0.05

6

35,994

V769_D770insASV

0.1

0.05

4

23,378

T790M

0.11

0.05

5

33,633

L858R

0.09

0.1

2

27,703

HD780 (0%)

30

570

32,314

2353

E746_A750delELREA

0

nd

0

 

V769_D770insASV

0

nd

0

 

T790M

0

nd

0

 

L858R

0

nd

0

 

HD780 (0.1%)

20

705

22,117

2734

E746_A750delELREA

0.03

0.05

1

29,492

V769_D770insASV

0.1

0.05

3

20,616

T790M

0.19

0.05

6

28,438

L858R

0.13

0.1

2

19,308

  1. RS Reference Standard, Median Read Cov Median Read Coverage, Median Mol Cov Median Molecular Coverage, VAF Variant Allele Frequency, LOD Limit of Detection, Allele Mol Cov Allele Molecular Coverage, Tot Read Cov Total Read Coverage, nd not detected, HD Horizon Discovery