Fig. 4

TRPM3 activity after stimulation with nifedipine alone. Data were obtained under whole-cell patch clamp conditions. a. A representative time-series of current amplitude at + 100 mV and − 100 mV showing the effect of 100 μΜ nifedipine on ionic currents in isolated NK cells from HC. b. I–V before and after nifedipine stimulation in a cell corresponding with (a.). c. A representative time-series of current amplitude at + 100 mV and − 100 mV showing the effect of 100 μΜ nifedipine on ionic currents in isolated NK cells from CFS/ME patients. d. I–V before and after nifedipine stimulation in a cell as shown in (c.). e Bar graphs representing TRPM3 current amplitude at + 100 mV after stimulation with 100 μΜ nifedipine in CFS/ME patients (N = 6; n = 25) compared with HC (N = 6; n = 23). Data are represented as mean ± SEM. Abbreviations: CFS/ME, chronic fatigue syndrome/myalgic encephalomyelitis; HC, healthy controls; NK, natural killer; PregS, Pregnenolone sulfate; TRPM3, Transient Receptor Potential Melastatin 3