Table 1 Primers used for qRT-PCR analysis
Genes | Primer sequences (5′-3′) |
|---|---|
hsa(mmu)-miR-122 | F: 5′-AGCGTGGAGTGTGACAATGG-3’ |
|  | R: 5′-GTCGTATCCAGTGCAGGGTCCGAG GTATTCGCACTGGATACGACCAAACA-3’ |
hsa(mmu)-U6 | F: 5′-CTCGCTTCGGCAGCACA-3’ |
|  | R: 5′-AACGCTTCACGAATTTGCGT-3’ |
hSirt1 | F: 5′-TGCCGGAAACAATACCTCCA-3’ |
|  | R: 5′-AGACACCCCAGCTCCAGTTA-3’ |
hSREBP1 | F: 5′-GGAGCCATGGATTGCACTTTCG-3’ |
|  | R: 5′-GCTCAGGAAGGCTTCAAGAGAG-3’ |
hFASN | F: 5′-TATGAAGCCATCGTGGACGG-3’ |
|  | R: 5′-GAAGAAGGAGAGCCGGTTGG-3’ |
hSCD1 | F: 5′-CTTGCGATATGCTGTGGTGC-3’ |
|  | R: 5′-AAGTTGATGTGCCAGCGGTA-3’ |
hACC1 | F: 5′-CAAGGTCAGCTGGTCCACATG-3’ |
|  | R: 5′-GTGGAATACCTTCTGCCCTAGC-3’ |
hApoA5 | F: 5′- GACCAGGAGACTGAGGAGGT-3’ |
|  | R: 5′-TTGCTCAGAACCTTGCCACT-3’ |
hβ-actin | F: 5′- CCTCGCCTTTGCCGATCC-3’ |
|  | R: 5′- GGATCTTCATGAGGTAGTCAGTC − 3’ |
mSirt1 | F: 5′-GACGCTGTGGCAGATTGTTA-3’ |
|  | R: 5′-GGAATCCCACAGGAGACAGA-3’ |
mSREBP1 | F: 5′-CCACAATGCCATTGAGAAGCG-3’ |
|  | R: 5′-CTGACACCAGGTCCTTCAGTG-3’ |
mFASN | F: 5′-TTGCTGGCACTACAGAATGC-3’ |
|  | R: 5′-AACAGCCTCAGAGCGACAAT-3’ |
mSCD1 | F: 5′-CACACCTTCCCCTTCGACTA-3’ |
|  | R: 5′-TGACTCCCGTCTCCAGTTCT-3’ |
mACC1 | F: 5′-CTTGGAGCAGAGAACCTTCG-3’ |
|  | R: 5′-ACTTCCCGACCAAGGACTTT-3’ |
mApoA5 | F: 5′-AGAAGCTGGCACAGGAGAAC-3’ |
|  | R: 5′-AGCTGAGCCTTGGTGTCTTC-3’ |
mβ-actin | F: 5′-GTCGTACCACAGGCATTGTGATGG-3’ |
|  | R: 5′-GCAATGCCTGGGTACATGGTGG-3’ |