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Fig. 2 | Molecular Medicine

Fig. 2

From: Astragalus polysaccharides suppresses high glucose-induced metabolic memory in retinal pigment epithelial cells through inhibiting mitochondrial dysfunction-induced apoptosis by regulating miR-195

Fig. 2

APS inhibited the high glucose-induced upregulation of miR-195. a Treatment with APS significantly decreased the expression of miR-195 and increased the expression of Bcl-2 which were induced by HG or HG + NG treatment in ARPE-19 cell line. b Treatment with APS significantly decreased the expression of miR-195 and increased the expression of Bcl-2 which were induced by HG or HG + NG treatment in PRPE cells. c Sequence analysis result showed miR-195 directly targeted to the 3’UTR of Bcl-2. d Dual luciferase reporter assay showed miR-195 repressed the luciferase activity which contained the WT Bcl-2 3’UTR, but not the mutant Bcl-2 3’UTR. Error bars represented mean ± SD. *** p < 0.001, ** p < 0.01, * p < 0.05

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Molecular Medicine

ISSN: 1528-3658