Fig. 2

Effect of the G186R variant on SHARPIN function. a NF-κB activity in HEK293 cells under TNF-α–induced activation was determined by luciferase assay, which was performed three times with five replicates in each assay. Myc: Myc vector; WT: wild-type Myc-SHARPIN; GR: G186R variant of Myc-SHARPIN. *: p < 0.005; **: p < 0.0005. b Localization of Myc-SHARPIN visualized by immunocytochemistry in HEK293 cells. c Magnified images of Myc-SHARPIN. The G186R variant showed granular accumulations of Myc-SHARPIN. Scale bar: 10 μm