Fig. 2

Deficiency in CIRP reversed the decreased regional metabolic activity in the WT mice after binge alcohol. a Regions identified by SPM analysis using two way ANOVA in which metabolic activity was decreased in WT mice but increased in KO mice during post-alcohol were aligned to mouse MRI brain template and regions anatomically identified based on a three dimensional mouse brain atlas (Paxinos and Franklin 2008). b Percent changes in globally normalized metabolic values decreased in WT mice but increased in CIRP^−/− (KO) mice over specific regions revealed by the two-way ANOVA. The regions are as follows: R1-Olfactory bulb, glomerular layer, R2-agranular insular cortex, ventral part, R3-cerebellum, 3rd lobule of cerebellar vermis, R4- posteromedial cortical amygdaloid area, R5-hippocampus, fimbria, R6-primary visual cortex, R7-primary somatosensory cortex, barrel field. See Table 2 for the stereotaxic coordinates and anatomic volumes of these regions. Data are expressed as mean ± SE; * P < 0.05, ** P < 0.005, KO vs. WT mice of respective regions