Fig. 6

circRNA-9119 targeted miR-26a and miR-136. a Graphical illustration of the conserved circRNA-9119-binding motifs in miR-26a and miR-136. b Luciferase function was evaluated with luciferase reporter products comprising either WT or mutated (MU) copy of human circRNA-9119 after transfection with miR-26a/miR-136 mimic. Luciferase function was normalized to β-galactosidase level. c and d Testes in experimental group received injection of 0.3-μg poly I:C, while the control group was injected with PBS. At various time points, testes were homogenized and examined for miR-26a and miR-136 expression by qRT-PCR. e and f Sertoli and Leydig cells were separated from C57BL/6 mice and transfected with circRNA-9119 siRNA or NC siRNA prior to treatment with poly I:C or PBS for 6 h. Total RNAs were isolated from cells, and miR-26a and miR-136 concentrations were evaluated with qRT-PCR after normalization to GAPDH level. Results are shown as means ± SEM (n = 3 replicates each experiment). *P < 0.05, **P < 0.01, ***P < 0.001