Fig. 9

CircRNA-9119-modulated testicular inflammatory reactions were in part attributed to TLR3 and RIG-I expression. Sertoli and Leydig cells were separated from C57BL/6 mice and transfected with circRNA-9119 siRNA or NC siRNA prior to treatment with poly I:C or PBS for 6 h. Total RNA and proteins were isolated from cells. Translation a and b and transcription c and d of TLR3 and RIG-I were investigated with western blotting and qRT-PCR, respectively. e and f Sertoli and Leydig cells were separated form WT C57BL/6, TLR3^−/−, and RIG-I^−/− mice before treatment with poly I:C or PBS (Ctrl) for 6 h. qRT-PCR was performed to examine the expression level of IL-1β, IFN-β, and MCP-1 in Leydig and Sertoli cells. Results are expressed as means ± SEM (n = 3 replicates each experiment). *P < 0.05, **P < 0.01 versus respective Ctrl group; ^#P < 0.05 versus poly I:C/WT group