Fig. 3

Flow cytometry analysis of the distribution of lymphocytes in the lamina propria of GF + PBS mice, GF + B6 mice and GF + SLE mice. (a) Histograms of CD19⁺ B cells. The number is the percentage of CD19⁺ B cells of live cells. The red histograms show CD19⁺ cells of live cells. The right statistical results show percentages of B cells in groups (5 mice per group). (b) Plots represent CD4⁺ CD8a⁻ T cells (left) and the percentages of CD4⁺ CD8a⁻ T cells in 3 groups (5 mice per group; right). (c) Plots represent CD4⁺ RORγt ⁺ T cells (left) and percentages of CD4⁺ RORγt ⁺ cells in 3 groups (5 mice per group; right). (d) Plots represent regulatory T cells (Tregs; left) and percentages of Treg cells in 3 groups (5 mice per group; right). Data represent one of three independent experiments. A Mann-Whitney t-test was used to compare groups, and error bars represent means ± SEMs. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001