Fig. 2

Targeted inhibition of YAP can suppress the proliferation and increase the chemosensitivity of EC cells. (a) Cells were transfected with siRNA negative control (si-NC) or si-YAP for 24 h, the expression of YAP was measured by western blot analysis (left) and quantitatively analyzed (right); Ishikawa (b) or AN3CA (c) cells were transfected with si-NC or si-YAP for the indicated time periods, the cell proliferation was measured by CCK-8 kit; (d) Ishikawa or AN3CA cells were treated with increasing concentrations of VP for 48 h, the cell proliferation was measured by CCK-8 kit; (e) Ishikawa cells were pre-treated with or without 1 μM of VP and then further treated with increasing concentrations of Dox for 48 h, the cell proliferation was measured by CCK-8 kit; (F) Ishikawa cells were pre-transfected with si-NC or si-YAP for 12 h and then further treated with increasing concentrations of Dox for 48 h, the cell proliferation was measured by CCK-8 kit. Data are presented as means ± SD of three independent experiments. *p < 0.05, ** p < 0.01 compared with control