Fig. 3

YAP can regulate the expression of IL-6 and IL-11 in EC cells. Ishikawa (a) or AN3CA (b) cells were transfected with si-NC or si-YAP for 24 h, the mRNA expression of cytokines was measured by qRT-PCR; (c) Ishikawa cells were treated with increasing concentrations of VP for 24 h, the mRNA expression of cytokines was measured by qRT-PCR; (d) Ishikawa cells were treated with or without 1 μM of VP for 24 h, the expression of cytokines was measured by ELISA; (e) Ishikawa cells were transfected with si-NC or si-YAP for 24 h, the expression of cytokine was measured by ELISA; Ishikawa cells were transfected with pcDNA vector or pcDNA/YAP for 24 h, (f) the protein expression of YAP was tested by western blot, (g) the mRNA expression of IL-6 and IL-11 was tested by qRT-PCR. *p < 0.05, ** p < 0.01 compared with control