Fig. 4

Both IL-6 and IL-11 are involved in YAP regulated malignancy of EC cells. After transfected with si-NC or si-YAP for 12 h, Ishikawa (a) or AN3CA (b) cells were further treated with rIL-6 (200 ng/ml) or rIL-11 (200 ng/ml) for 48 h. The cells proliferation was tested by CCK-8 kit; (c) After pre-treated with or without VP (5 μM) for 1 h, Ishikawa cells were further treated with rIL-6 (200 ng/ml) or rIL-11 (200 ng/ml) for 48 h; (d) After pretreated with or without Dox (1 μM) and/or VP (1 μM) for 1 h, Ishikawa cells were further treated with rIL-6 (200 ng/ml) or rIL-11 (200 ng/ml) for 48 h. Data are presented as means ± SD of three independent experiments. *p < 0.05, ** p < 0.01 compared with control