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Fig. 9 | Molecular Medicine

Fig. 9

From: Melatonin prevents bone destruction in mice with retinoic acid–induced osteoporosis

Fig. 9

Melatonin can inhibit osteoclast differentiation and promote osteogenic differentiation in vitro. RANKL-induced RAW264.7 cells were cultured with 1 μM retinoic acid (RA) in the presence or absence of 100 nM melatonin. MC3T3-E1 cells were cultured with osteogenic medium with or without RA in the presence or absence of 100 nM melatonin. RAW264.7 cells were stained with TRACP and the number of TRACP-stained osteoclasts was quantified (a). The expression levels of Calcr, Ctsk, Rank, Nox1, Nox2, Sod1, and Sod2 were examined by RT-PCR and western blot (b, c, f and g). Then, the expression levels of Runx2, Ocn, Opn, Nox1, Nox2, Sod1 and Sod2 in MC3T3-E1 cells were examined by qRT-PCR and Western blot (d, e, i and j). Signaling pathway related proteins (p-p65/p65, p-IκBα/IκBα, p-Erk/Erk, and p-Smad1/Smad1) were examined by western blot (h and k). Control: RANKL-induced RAW264.7 and osteogenic medium induced MC3T3-E1 cells; RA: RA-induced cells; RA + Mlt: melatonin-treated cells. *, P < 0.05 vs control. #, P < 0.05 vs RA

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