Fig. 4

Quantification of TAN, TAN', and TAN + TAN' by CE-TOFMS. a Quantitative CE-TOFMS data for TAN coincided with the HPLC results. b TAN' was increased at ischemic state in contrast to the decrease in TAN, and decreased again after reperfusion. c The values of TAN+TAN’ did not significantly differ at stationary state among all groups and were well preserved at ischemic state; nevertheless, this parameter was decreased to as low as one half of that in the stationary state in the reperfused phase. d ΔhTAN + TAN' appeared to be lower in the febusostat and topiroxostat groups than in the vehicle and allopurinol groups. TAN = ATP + ADP + AMP), TAN = dATP + phosphoribosyl diphosphate (PRPP) + adenosine + adenine + inosine + IMP + hypoxanthine + xanthine + uric acid, ΔhTAN + TAN' was calculated as the TAN + TAN' of the reperfused state minus that of the ischemic state. Concentrations are expressed as nmol/g wet weight; the data are presented as the mean ± SEM (n = 5/6). *P < 0.05 and **P < 0.01 versus Veh; $P < 0.05 versus Feb; #P < 0.05 and ##P < 0.01 versus Allo; one-way ANOVA followed by Games–Howell’s test