Fig. 8

Immunohistochemistry for the detection of macrophages (ED1) and T-cells (CD3) (200x). Only a few ED1+ cells were present in the normal peritoneal membrane (a). CKD induced a significant number of macrophages in the peritoneum (b). However, the largest number of macrophages was detected in the thickened peritoneal membrane of the PF (c) and CKD/PF groups (d). TAM (e) and rBMP7 (f) protected the peritoneal membrane against inflammatory infiltrate comprising macrophages, as shown by quantitative analysis (m). A few positive T-cells were detected in the normal peritoneal membrane (g). T-cells were also observed in the CKD (h) and PF groups (i). An important number of T-cells were detected in the CKD/PF group (j). TAM (k), and rBMP7 (l) treatments decreased the number of T-cells in the peritoneal membrane (n). Overall ANOVA p < 0.0001. Post-test p-values: *p < 0.01 vs Control; ^#p < 0.01 vs CKD, ^†p < 0.01 vs PF, ^§p < 0.01 vs PF/CKD