Fig. 2
From: PRSS1 mutation: a possible pathomechanism of pancreatic carcinogenesis and pancreatic cancer
PRSS1_R116C mutation promotes the malignant phenotype of PANC-1 cells. a. The transwell chamber system was applied to assess the effect of PRSS1 wild type/R116C mutant overexpression on PANC-1 cell migration (converted microscope, 200×); b. The matrigel assay was performed to evaluate the effect of PRSS1 wild type/R116C mutant overexpression on PANC-1 cell invasion; c. CCK8 assay was used to measure PANC-1 cell proliferation; d. Effect of PRSS1 wild type/R116C mutant overexpression on PANC-1 cell cycle distribution; e. Flow cytometry was employed to measure the effect of PRSS1 wild type/R116C mutant on PANC-1 cell proliferation; f. R116C mutation prominently increased ERK and VEGFR2 expression in pancreatic cancer