Fig. 2

Increased PMNL migration upon CC16 neutralization is associated with concurrently enhanced CD62L. a Gating strategy of isolated PMNL and representative stainings for isotype or anti CD11b/CD18, CD62L and CD31 antibodies for the MFU evaluation is shown. Migratory rate of PMNL and CD11b/CD18, CD31, CD62L expression on PMNL after their incubation with serum samples from severely injured trauma patients. Patients were grouped to no P group without pneumonia or P group with pneumonia. Samples were obtained at admission to emergency department (ED) and one (Sadeghi-Bazargani et al. 2018) day prior to pneumonia. Samples from the equal post-injury days in the corresponding no P group were used. b Migratory rates of PMNL isolated from healthy volunteers towards IL-8 or sera from trauma patients with or without CC16 neutralization (aCC16-AB) is shown. c CD11b/CD18 expression, (d) CD62L expression and (e) CD31 expression on neutrophils is shown. Data are represented as mean ± SEM. p < 0.05: §: vs. all; *: vs. ctrl; Ф: P vs. corresponding no P group; #: CC16 vs. indicated