Fig. 3

CC16-induced oxidative burst notably in the group of traumatized patients who develop complications is associated with CXCR1 and CXCR2 in mature neutrophils. a Gating strategy of isolated PMNL and representative stainings for CD16 and subsequent gating of CD16CD62L subsets with the exemplary MFU evaluation is shown. b Oxidative stress rate of PMNL after their incubation with IL-8 or sera samples from severely injured trauma patients. Patients were grouped to no P group without pneumonia or P group with to pneumonia. Samples were obtained at admission to emergency department (ED) and one (Sadeghi-Bazargani et al. 2018) day prior pneumonia. Samples from the equal post-injury days in the corresponding no P group were used. c Oxidative burst activity in CD16⁺ granulocytes after incubation with CC16 and neutralization of CXCR1 (CXCR1-ab), CXCR2 (CXCR2-ab) or the corresponding isotype control antibodies (ctrl-ab), respectively. d Oxidative burst activity of immature CD16^dimCD62L^bright, (e) mature CD16^brightCD62L^bright and (f) immunesuppressive CD16^brightCD62L^dim granulocytes. Data are represented as mean ± SEM. p < 0.05: *: vs. ctrl; #: CC16 vs. indicated