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Fig. 1 | Molecular Medicine

Fig. 1

From: Inhibition of phosphatidylinositol 3-kinase by PX-866 suppresses temozolomide-induced autophagy and promotes apoptosis in glioblastoma cells

Fig. 1

Temozolomide induces autophagic flux in glioma cells. a T98G cells were treated with increasing doses of TMZ for 48 h and only living (attached) cells were collected for analysis. Western blotting shows an increase in LC3 II protein levels and a decrease in p62 protein levels. GAPDH was used as a loading control. b The LC3 flux assay was performed on T98G cells. T98G cells were treated with vehicle or TMZ (500 μM) for 48 h containing either DMSO or Baf (10 nM) and only living (adherent) cells were collected. Western blotting for LC3 indicates an increase in LC3 II for the TMZ + Baf lane compared to Baf alone, proving TMZ-induced autophagosome formation. The data (LC3 II/GAPDH) was quantified and graphed to mark this increase. Data represents three independent experiments

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Molecular Medicine

ISSN: 1528-3658