Fig. 5
rhMFG-E8 is associated with reduced apoptosis in CLP after acute alcohol exposure. Early apoptotic cells in the thymus, (a) assessed by flow cytometry (lower right quadrant, Q4; representative image) (b) and quantified, and apoptosis in the (c) spleen, assessed using caspase 3 activity, were increased rats subjected to CLP and treated with vehicle (human albumin) compared with sham surgery, and lower in rats subjected to CLP and treated with rhMFG-E8. Prior to CLP, all rats were exposed to acute alcohol intoxication; means + SE; n = 10–14 for sham and n = 9 for other experimental groups; ANOVA plus Student-Newman-Keuls method, * P < 0.05 vs. Sham, # P < 0.05 vs. Vehicle