Fig. 3

The p-Akt, p-Bad and miR-320 expressions were observed in different groups. a: The p-Akt and p-Bad were determined by immunohistochemistry assay. b: The expressions of PTEN, p-Akt, p-Bad, Akt and Bad were detected by Western blotting. c: ED decreased PTEN expression but increased p-Akt and p-Bad expressions. d: The p-Akt/Akt level in different groups. e: The p-Bad/Bad level in different groups. F: The miR-320 expression was determined by quantitative real-time polymerase chain reaction (qRT-PCR). ^**P < 0.01 vs. Sham, ^##P < 0.01 vs. Model