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Fig. 4 | Molecular Medicine

Fig. 4

From: Edaravone reduces oxidative stress and intestinal cell apoptosis after burn through up-regulating miR-320 expression

Fig. 4

The apoptosis and the target gene of miR-320 were determined. a: The miR-320 expression was determined by qRT-PCR. b: The apoptosis was detected by flow cytometry. c: The apoptosis was up-regulated after inhibited miR-320 expressions. d: The Bcl-2, cytochrome c, C caspase-9 and C caspase-3 expressions were detected by Western blotting. e: After the inhibition of miR-320 expression, the expressions of cytochrome c, C caspase-9 and C caspase-3 were increased and the expression of Bcl-2 was decreased. f: The miR-320 potential target gene was predicted by Targetscan 7.2. g: The PTEN expression was detected by Western blotting. h: The PTEN expression in antago group was up-regulated. **P < 0.01 vs. ED, ##P < 0.01 vs. Scrambled

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