Fig. 1
Skeletal changes and growth retardation in Sp7-Cre;SMS1f/f;SMS2−/− mice. a Gross appearance of C57BL/6, SMS1f/f;SMS2−/−, Sp7-Cre;SMS1f/f;SMS2−/− and Sp7-Cre mice at p1. Sp7-Cre;SMS1f/f;SMS2−/− mice are small. Bar = 1 cm. b Skeletons from E15.5-E17.5 embryos and newborns (p1) of C57BL/6, SMS1f/f;SMS2−/−, Sp7-Cre;SMS1f/f;SMS2−/− and Sp7-Cre mice. Embryos and newborns were stained with Alizarin red and Alcian blue. Note the delayed mineralization of the limbs in the Sp7-Cre;SMS1f/f;SMS2−/− E15.5 embryo compared with that of C57BL/6 and SMS1f/f;SMS2−/− mice (black square). Dorsal views of skulls of newborns showed that calvarial bone was hypomineralized in Sp7-Cre;SMS1f/f;SMS2−/− mice. c Gross morphology of C57BL/6, SMS1f/f;SMS2−/−, Sp7-Cre;SMS1f/f;SMS2−/−, Sp7-Cre;SMS1+/+;SMS2+/−, and Sp7-Cre;SMS1+/+;SMS2−/− male mice at 15 weeks after birth. d Body weight of C57BL/6, SMS1f/f;SMS2−/−, Sp7-Cre;SMS1f/f;SMS2−/−, Sp7-Cre;SMS1+/+;SMS2+/−, and Sp7-Cre;SMS1+/+;SMS2−/− male mice at indicated time points (n = 10 for each genotype). *p < 0.05 versus C57BL/6 and SMS1f/f;SMS2−/−. **p < 0.05 versus C57BL/6, SMS1f/f;SMS2−/−, Sp7-Cre;SMS1+/+;SMS2−/−, and Sp7-Cre;SMS1+/+;SMS2+/−. Data are mean ± SD