Fig. 2

Note the osteoporotic phenotype of the Sp7-Cre;SMS1^f/f;SMS2^−/− mice. a Representative micro-CT images of the distal femoral shafts of 15-week-old C57BL/6, SMS1^f/f;SMS2^−/−, Sp7-Cre;SMS1^+/+;SMS2^+/−, Sp7-Cre;SMS1^+/+;SMS2^−/− and Sp7-Cre;SMS1^f/f;SMS2^−/− mice. 3D reconstruction of trabecular bone. b Micro-CT analysis of femurs from 15-week-old mice. Abbreviations: BV, bone volume; BV/TV, bone volume/total volume; Tb N, trabecular number; Tb Sp, trabecular separation; *p < 0.05 versus C57BL/6, SMS1^f/f;SMS2^−/−, Sp7-Cre;SMS1^+/+;SMS2^+/− and Sp7-Cre;SMS1^+/+;SMS2^−/−; mean ± SD, n = 10 for each genotype. c Isolated femurs of 15-week-old mice were scanned using pQCT. Sections were made at the midshaft. d Quantification of cortical density, trabecular density, cortical thickness and strength strain index (SSI). *p < 0.005 versus C57BL/6, SMS1^f/f;SMS2^−/−, and Sp7-Cre;SMS1^+/+;SMS2^−/−, **p < 0.05 versus C57BL/6, SMS1^f/f;SMS2^−/− and Sp7-Cre;SMS1^+/+;SMS2^−/−; mean ± SD, n = 8–10 for each genotype