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Fig. 4 | Molecular Medicine

Fig. 4

From: Deficiency of sphingomyelin synthase 1 but not sphingomyelin synthase 2 reduces bone formation due to impaired osteoblast differentiation

Fig. 4

Effects of SMS1 knock-out on osteoblast cell growth. a Schematic outline of the experimental design. Calvarial osteoblast cells were seeded on 48-well plates (5000 cells/well) with 10% FBS containing α-MEM. b Reduction of expression of SMS1 in osteoblasts treated with 1 μM 4-hydroxy tamoxifen (4-OHT). Primary osteoblast cells were isolated from the calvaria of newborn ERT2-Cre;SMS1f/f;SMS2−/− mice. c The number of cells was counted 4 days after treatment with 4-OHT. Inactivation of SMS1 resulted in no growth inhibition of calvarial osteoblasts. Treatment with 4-OHT does not affect the cell growth of primary SMS1f/f;SMS2−/− calvarial osteoblasts. Osteoblasts were prepared from newborn SMS1f/f;SMS2−/− and ERT2-Cre;SMS1f/f;SMS2−/− mice. Values are expressed as the mean ± SD, n = 8 for each genotype

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Molecular Medicine

ISSN: 1528-3658