Skip to main content
Fig. 8 | Molecular Medicine

Fig. 8

From: Retinal pigment epithelium degeneration caused by aggregation of PRPF31 and the role of HSP70 family of proteins

Fig. 8

Mobility of PRPF31 protein to the nucleus is affected by the p.A216P mutation. ARPE-19 cells transfected with the pEGFP-N1 plasmid alone or carrying the open reading frame of human WT PRPF31 (PRPF31-GFP) or carrying the point mutation p.A216P (A216P-GFP) were imaged − 3 s pre-photobleaching (a, e, i) and 0 s (b, f, j), 100 s (c, g, k) and 200 s (d, h, l) post-photobleaching. The recovery curve (m) indicates that the cells transfected with mutant A216P-GFP have a smaller mf than those transfected with PRPF31-GFP (n). The boxplot n represents the normalized fluorescence intensity and mobile fraction in PRPF31-GFP and A216P-GFP transfected ARPE-19 cells (n = 5 in each group). Statistically significant differences were determined by Mann-Whitney U-test (*p < 0.05). Fi = fluorescence before bleaching, F0 = fluorescence just after bleaching, F∞ = fluorescence in the bleached region after full recovery. Scale bars represent 10 μm

Back to article page