Fig. 5

NEAT1 and Akt signaling downregulation were involved in the shikonin against paclitaxel-resistant NSCLC. a Either low dose or high dose of shikonin considerably suppressed the NEAT1 knockdown A549/PTX cell growth. b Either low dose or high dose of shikonin considerably induced the cell apoptotic death in NEAT1 knockdown A549/PTX cells. c p-Akt expression was decreased, cleaved PARP and cleaved caspase-3 expression were increased after shikonin treatment in NEAT1 knockdown A549/PTX cells. d The proliferation of NEAT1 overexpression A549/PTX cells was not inhibited after shikonin treatment. e Apoptosis rates were not enhanced after shikonin treatment. f Shikonin could not decrease p-Akt expression, also could not increase cleaved PARP and cleaved caspase-3 expression after shikonin treatment in NEAT1 knockdown A549/PTX cells. *Statistically significant difference (p < 0.05). #No statistically significant difference(p > 0.05)