Fig. 1From: Vitamins D3 and D2 have marked but different global effects on gene expression in a rat oligodendrocyte precursor cell lineExperimental design of cell differentiation and treatment with vitamin D3. Cells were plated and cultured overnight before switching to DM. Vitamin D3 (Vit D3, 100 nM) or vehicle (control; Ctr) was added simultaneously with DM (a) or after 48 h (b). VDR gene expression was measured by RT-qPCR 24 h or 72 h after vitamin D3 or vehicle addition. Data are expressed as fold change (FC) versus Ctr1 and are the mean ± SD of four replicates. *** P < 0.001, **** P < 0.0001 by Student’s t-test. QPCR analysis was not conducted on D2-treated culturesBack to article page