Fig. 3

HULC served as a ceRNA for miR-372-3p in osteosarcoma. The miR-372-3p mimics and luciferase reporter plasmids with wild-type or mutant HULC 3′-UTR were co-transfected into U2OS cells. A dual luciferase reporter assay was performed to explore the direct binding relationship between HULC and miR-372-3p (A). U2OS cells were transfected with HULC-targeting shRNA (sh-HULC), negative control shRNA (sh-NC), pcDNA3.1-HULC overexpression vector (pcDNA-HULC) or empty vector plasmid (pcDNA-3.1), and then qRT-PCR was performed to evaluate the relative expression levels of HULC and miR-372-3p (B). Data are shown as the mean ± SD. * p < 0.05, ** p < 0.01