Fig. 2
From: Down-regulated miR-495 can target programmed cell death 10 in ankylosing spondylitis
Methylation detection of miR-495 promoter region. All data was presented as the mean + SD. a Sequence schema of the bisulfite-specific PCR sequenced region in the miR-495 promoter region. Numbers (1–10) indicate potential CpG islands. b Methylation status of CpGs in the miR-495 promoter region. White and black circles indicated un-methylated and methylated CpGs, respectively. AS1, AS2, AS3, AS4: different patients with AS. HC1, HC2, HC3, HC4: different healthy controls. c The percentage of miR-495 promoter methylation. The percentage of miR-495 promoter methylation in healthy controls and patients with AS was 0.79 + 3.30 and 0.18 + 2.63, respectively. d MeDIP-qPCR of GADPH, miR-495, and PDCD10 promoters. The values of GADPH promoter methylation in healthy controls and patients with AS were 1.01 + 0.025 and 1.10 + 0.064, respectively. The values of miR-495 promoter methylation in healthy controls and patients with AS were 0.97 + 0.036 and 6.68 + 0.224, respectively. The values of PDCD10 promoter methylation in healthy controls and patients with AS were 0.93 + 0.11, and 0.94 + 0.028, respectively. n = 150 or 20: tested samples in each group. AS: patients with ankylosing spondylitis. HC: healthy controls. ***: P value < 0.01