Fig. 4
From: TFPI2 suppresses breast cancer progression through inhibiting TWIST-integrin α5 pathway
TFPI2 regulated TWIST1 expression in breast cancer. a Down-regulation of TFPI2 in MCF7 cells transfected with shTFPI2#1. #2, #3 indicated by qRT-PCR. ** represents shTFPI2#1. #2, #3 vs. shNC, p < 0.01. b Over-expression of TFPI2 inhibited protein expression of TWIST1 and integrin α5, while inhibition of TFPI2 promoted TWIST1 and integrin α5. c Immunohistochemistry of TWIST1 in Normal and Tumor breast cancer tissues, suggesting up-regulation of TWIST1 in tumor tissues. d Negative correlation TFPI2 and TWIST1 in breast cancer patients